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interleukin 10 il 10 monoclonal antibody  (Proteintech)


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    Proteintech interleukin 10 il 10 monoclonal antibody
    Detection results <t>of</t> <t>IL-10</t> in skin wound area; NC: regular control group; PN: Panax notoginseng group; A(1, 2)∼F(1, 2): Immunohistochemical staining of IL-10 in skin wound area; G: relative mRNA expression level of IL-10; H: IL-10 protein expression level detected by ELISA; Comparison between PN and NC, * P<0.05; **P<0.01
    Interleukin 10 Il 10 Monoclonal Antibody, supplied by Proteintech, used in various techniques. Bioz Stars score: 96/100, based on 355 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/interleukin+10+il+10+antibodies/IL-10+Antibody/bio_rxiv__64898__2026__02__28__708691-65-19-26
    Average 96 stars, based on 355 article reviews
    interleukin 10 il 10 monoclonal antibody - by Bioz Stars, 2026-10
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    Images

    1) Product Images from "Exploring the mechanism of Panax Notoginseng in the treatment of skin wound based on network pharmacology and experimental verification"

    Article Title: Exploring the mechanism of Panax Notoginseng in the treatment of skin wound based on network pharmacology and experimental verification

    Journal: bioRxiv

    doi: 10.64898/2026.02.28.708691

    Detection results of IL-10 in skin wound area; NC: regular control group; PN: Panax notoginseng group; A(1, 2)∼F(1, 2): Immunohistochemical staining of IL-10 in skin wound area; G: relative mRNA expression level of IL-10; H: IL-10 protein expression level detected by ELISA; Comparison between PN and NC, * P<0.05; **P<0.01
    Figure Legend Snippet: Detection results of IL-10 in skin wound area; NC: regular control group; PN: Panax notoginseng group; A(1, 2)∼F(1, 2): Immunohistochemical staining of IL-10 in skin wound area; G: relative mRNA expression level of IL-10; H: IL-10 protein expression level detected by ELISA; Comparison between PN and NC, * P<0.05; **P<0.01

    Techniques Used: Control, Immunohistochemical staining, Staining, Expressing, Enzyme-linked Immunosorbent Assay, Comparison

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    Article Snippet: Diabetic wounds significantly impair the life quality of patients and impose substantial economic burdens on both individuals and healthcare systems.. Hydrogel dressings, owing to their multifunctional properties, have emerged as a promising strategy for wound management.. By integrating gelation capabilities with inherent pharmacological activities, natural products are frequently employed in the development of hydrogel formulations.



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    Animal study verifying the effect of AE in mitigating EBI of SAH. (A) The complete timeline of an experimental plan, schematic diagram, and actual skull base image of the SAH/Sham model in this animal experiment. Some elements are created in BioRender (2026); https://BioRender.com/vwwir98 . (B) SAH grading score of each group in this stage. n = 24. (C) Modified Garcia score of each group, n = 24. (D) The water content of the left and right brains in different groups at this stage. n = 6. (E and F) Apoptosis of bleeding focus cortical neurons was detected by TUNEL assay (red), NeuN (green), and DAPI (blue) immunofluorescence. n = 6. Scale bars, 50 μm. (G) Bars show changes in BBs in SAH mice. n = 24. (H to N) Western blotting images and quantitative data of relative expression level of Il-1b, Il-6, Nos2, <t>Il-4,</t> <t>Il-10,</t> and Tgfb1 in the ipsilateral cortex after SAH. n = 6. (O) The coronal sections were stained with hematoxylin and eosin (H&E) for histological evaluation AE-regulated in SAH. n = 6. Scale bars, 50 μm. (P) Histopathological changes detected by Nissl’s staining in hippocampus and the hemorrhagic cortex. n = 6. Scale bars, 50 μm. In (B), (C), and (G), data were represented as median (interquartile range), and P values were calculated using Kruskal–Wallis H with Dunn test. In (D), (F), and (I) to (N), data were represented as mean ± SD, and P values were calculated using one-way ANOVA with Tukey multiple comparisons. * P < 0.05, ** P < 0.01, *** P < 0.001. The original Western blot images are shown in Data . Detailed experimental data and statistical results, including Z and P values for Kruskal–Wallis test and F and P values for ANOVAs, are provided in Data to .
    Rabbit Anti Interleukin 10 Il 10 Polyclonal Antibody, supplied by ABclonal Biotechnology, used in various techniques. Bioz Stars score: 98/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Identification of IGSF8 and <t>IL10RB</t> as new ADAM9 substrates. HCT116 cells were transfected with the indicated siRNA or expression constructs, and Western blotting was performed for conditioned media (CM) or cell lysates (CLs) using antibodies recognizing the ectodomain of IGSF8 ( A , B ) or IL10RB ( C , D ). Three biological replicates are shown in ( A , C ); unpaired two-tailed t test was performed. ADAM, A Disintegrin And Metalloproteinase; IGSF8, immunoglobulin superfamily member 8; IL10RB, interleukin 10 receptor subunit beta.
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    interleukin 10 il 10 monoclonal antibody  (Proteintech)
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    Image Search Results


    Animal study verifying the effect of AE in mitigating EBI of SAH. (A) The complete timeline of an experimental plan, schematic diagram, and actual skull base image of the SAH/Sham model in this animal experiment. Some elements are created in BioRender (2026); https://BioRender.com/vwwir98 . (B) SAH grading score of each group in this stage. n = 24. (C) Modified Garcia score of each group, n = 24. (D) The water content of the left and right brains in different groups at this stage. n = 6. (E and F) Apoptosis of bleeding focus cortical neurons was detected by TUNEL assay (red), NeuN (green), and DAPI (blue) immunofluorescence. n = 6. Scale bars, 50 μm. (G) Bars show changes in BBs in SAH mice. n = 24. (H to N) Western blotting images and quantitative data of relative expression level of Il-1b, Il-6, Nos2, Il-4, Il-10, and Tgfb1 in the ipsilateral cortex after SAH. n = 6. (O) The coronal sections were stained with hematoxylin and eosin (H&E) for histological evaluation AE-regulated in SAH. n = 6. Scale bars, 50 μm. (P) Histopathological changes detected by Nissl’s staining in hippocampus and the hemorrhagic cortex. n = 6. Scale bars, 50 μm. In (B), (C), and (G), data were represented as median (interquartile range), and P values were calculated using Kruskal–Wallis H with Dunn test. In (D), (F), and (I) to (N), data were represented as mean ± SD, and P values were calculated using one-way ANOVA with Tukey multiple comparisons. * P < 0.05, ** P < 0.01, *** P < 0.001. The original Western blot images are shown in Data . Detailed experimental data and statistical results, including Z and P values for Kruskal–Wallis test and F and P values for ANOVAs, are provided in Data to .

    Journal: Research

    Article Title: Aerobic Exercise-Induced TGF-β Receptor Reprogramming Disrupts Neutrophil–Microglia Crosstalk to Attenuate Early Brain Injury after Subarachnoid Hemorrhage

    doi: 10.34133/research.1301

    Figure Lengend Snippet: Animal study verifying the effect of AE in mitigating EBI of SAH. (A) The complete timeline of an experimental plan, schematic diagram, and actual skull base image of the SAH/Sham model in this animal experiment. Some elements are created in BioRender (2026); https://BioRender.com/vwwir98 . (B) SAH grading score of each group in this stage. n = 24. (C) Modified Garcia score of each group, n = 24. (D) The water content of the left and right brains in different groups at this stage. n = 6. (E and F) Apoptosis of bleeding focus cortical neurons was detected by TUNEL assay (red), NeuN (green), and DAPI (blue) immunofluorescence. n = 6. Scale bars, 50 μm. (G) Bars show changes in BBs in SAH mice. n = 24. (H to N) Western blotting images and quantitative data of relative expression level of Il-1b, Il-6, Nos2, Il-4, Il-10, and Tgfb1 in the ipsilateral cortex after SAH. n = 6. (O) The coronal sections were stained with hematoxylin and eosin (H&E) for histological evaluation AE-regulated in SAH. n = 6. Scale bars, 50 μm. (P) Histopathological changes detected by Nissl’s staining in hippocampus and the hemorrhagic cortex. n = 6. Scale bars, 50 μm. In (B), (C), and (G), data were represented as median (interquartile range), and P values were calculated using Kruskal–Wallis H with Dunn test. In (D), (F), and (I) to (N), data were represented as mean ± SD, and P values were calculated using one-way ANOVA with Tukey multiple comparisons. * P < 0.05, ** P < 0.01, *** P < 0.001. The original Western blot images are shown in Data . Detailed experimental data and statistical results, including Z and P values for Kruskal–Wallis test and F and P values for ANOVAs, are provided in Data to .

    Article Snippet: The membrane was blocked at room temperature for 1 h with 5% skim milk or bovine serum albumin, and then incubated overnight at 4 °C with the following primary antibodies: Rabbit anti-Lrg1 polyclonal antibody (Proteintech, 13224-1-AP, diluted 1:100); Rabbit anti-Il-1b polyclonal antibody (Baijia Biotechnology Co., Ltd., IPB0002); Rabbit anti-interleukin 6 (Il-6) polyclonal antibody (Baijia Biotechnology Co., Ltd., IPB0062); Rabbit anti-interleukin 4 (Il-4) polyclonal antibody (Baijia Biotechnology Co., Ltd., IPB3904); Rabbit anti-interleukin 10 (Il-10) polyclonal antibody (Baijia Biotechnology Co., Ltd., IPB0104); Rabbit anti-Tgfb1 polyclonal antibody (ABclonal, A16640); Rabbit anti-nitric oxide synthase 2 (Nos2) polyclonal antibody (Proteintech, 22226-1-AP); Rabbit anti-glyceraldehyde-3-phosphate dehydrogenase (GAPDH) polyclonal antibody (Proteintech, 10494-1-AP); Rabbit anti-Alk1 polyclonal antibody (Huabio, HA722529); Rabbit anti-Alk5 polyclonal antibody (Immunoway, YM8858); Rabbit anti-Tgfbr2 polyclonal antibody (Immunoway, YM8220); Rabbit anti-Eng polyclonal antibody (Proteintech, 10862-1-AP); Rabbit anti-Mothers Against Decapentaplegic Homolog 1/5 (Smad1/5) polyclonal antibody (immunoway, YT4325); Rabbit anti-Smad2/3 polyclonal antibody (Wanleibio, wl01520); Rabbit anti-phospho-Smad1/5 (p-Smad1/5) polyclonal antibody (Huabio, HA722566); Rabbit anti-p-Smad2/3 polyclonal antibody (Wanleibio, WL02305); Rabbit anti-DYKDDDDK-tag monoclonal antibody (Proteintech, 20543-1-AP); Rabbit anti-His-tag polyclonal antibody (Proteintech, 10001-0-AP); Rabbit anti-HA-Tag(26D11) polyclonal antibody (Proteintech, 51064-2-AP); Rabbit anti-myelocytomatosis oncogene (Myc)-tag monoclonal antibody (Abcom, ab9106); Rabbit anti-glutathione S-transferase (GST)-tag monoclonal antibody (ABclonal, AE006); and Rabbit anti-V5-tag polyclonal antibody (Proteintech, 14440-1-AP).

    Techniques: Modification, TUNEL Assay, Immunofluorescence, Western Blot, Expressing, Staining

    Primary peripheral neutrophil and microglia interactions based on wild-type and knockout mice. (A) Western blotting images demonstrated the effect of SB431541 treatment on the activation status of the TGF-β signaling pathway in SAH regulated by AE. (B to G) Quantitative analysis of relative expression level changes in Alk1, Alk5, Smad1/5, Smad2/3, p-Smad1/5, and p-Smad2/3 in the AE-regulated ipsilateral cortex in SAH, with the treat of SB431541. n = 6. (H) Schematic diagram of coculture experiments between primary peripheral neutrophils and primary microglia. (I) Immunofluorescent staining of primary microglia from wt/ko mice cocultured with primary neutrophils from wt/ko mice for Lpl/Sec24a (red), Iba1 (green), and DAPI (blue). Scale bars, 10 μm. (J) Immunofluorescent staining of primary peripheral neutrophils from wild-type and knockout mice for Lrg1 (red), Ly6G (green), and DAPI (blue). Scale bars, 10 μm. (K) Western blotting images of primary microglia from wt/ko mice cocultured with primary neutrophils from wt/ko mice on pro-inflammatory and anti-inflammatory factors and TGF-β signaling pathway. (L to W) Quantitative analysis of relative expression level of IL-1β, IL-6, Nos2, IL-4, IL-10, Tgfb1, Alk1, Alk5, Smad1/5, Smad2/3, p-Smad1/5, and p-Smad2/3 in the primary microglia cocultured with peripheral neutrophils. n = 3. (X) Quantitative analysis of the proportion of Lpl+/Iba1+ cells in the immunofluorescent staining of primary microglia in the primary cell coculture experiment. n = 3. (Y) Quantitative analysis of the proportion of Sec24a+/Iba1+ cells in the immunofluorescent staining of primary microglia in the primary cell coculture experiment. n = 3. In (B) to (G) and (L) to (Y), data were represented as mean ± SD, and P values were calculated using one-way ANOVA with Tukey multiple comparisons. * P < 0.05, ** P < 0.01, *** P < 0.001. The original Western blot images are shown in Data . Detailed experimental data and statistical results, including F and P values for ANOVAs, are provided in Data to .

    Journal: Research

    Article Title: Aerobic Exercise-Induced TGF-β Receptor Reprogramming Disrupts Neutrophil–Microglia Crosstalk to Attenuate Early Brain Injury after Subarachnoid Hemorrhage

    doi: 10.34133/research.1301

    Figure Lengend Snippet: Primary peripheral neutrophil and microglia interactions based on wild-type and knockout mice. (A) Western blotting images demonstrated the effect of SB431541 treatment on the activation status of the TGF-β signaling pathway in SAH regulated by AE. (B to G) Quantitative analysis of relative expression level changes in Alk1, Alk5, Smad1/5, Smad2/3, p-Smad1/5, and p-Smad2/3 in the AE-regulated ipsilateral cortex in SAH, with the treat of SB431541. n = 6. (H) Schematic diagram of coculture experiments between primary peripheral neutrophils and primary microglia. (I) Immunofluorescent staining of primary microglia from wt/ko mice cocultured with primary neutrophils from wt/ko mice for Lpl/Sec24a (red), Iba1 (green), and DAPI (blue). Scale bars, 10 μm. (J) Immunofluorescent staining of primary peripheral neutrophils from wild-type and knockout mice for Lrg1 (red), Ly6G (green), and DAPI (blue). Scale bars, 10 μm. (K) Western blotting images of primary microglia from wt/ko mice cocultured with primary neutrophils from wt/ko mice on pro-inflammatory and anti-inflammatory factors and TGF-β signaling pathway. (L to W) Quantitative analysis of relative expression level of IL-1β, IL-6, Nos2, IL-4, IL-10, Tgfb1, Alk1, Alk5, Smad1/5, Smad2/3, p-Smad1/5, and p-Smad2/3 in the primary microglia cocultured with peripheral neutrophils. n = 3. (X) Quantitative analysis of the proportion of Lpl+/Iba1+ cells in the immunofluorescent staining of primary microglia in the primary cell coculture experiment. n = 3. (Y) Quantitative analysis of the proportion of Sec24a+/Iba1+ cells in the immunofluorescent staining of primary microglia in the primary cell coculture experiment. n = 3. In (B) to (G) and (L) to (Y), data were represented as mean ± SD, and P values were calculated using one-way ANOVA with Tukey multiple comparisons. * P < 0.05, ** P < 0.01, *** P < 0.001. The original Western blot images are shown in Data . Detailed experimental data and statistical results, including F and P values for ANOVAs, are provided in Data to .

    Article Snippet: The membrane was blocked at room temperature for 1 h with 5% skim milk or bovine serum albumin, and then incubated overnight at 4 °C with the following primary antibodies: Rabbit anti-Lrg1 polyclonal antibody (Proteintech, 13224-1-AP, diluted 1:100); Rabbit anti-Il-1b polyclonal antibody (Baijia Biotechnology Co., Ltd., IPB0002); Rabbit anti-interleukin 6 (Il-6) polyclonal antibody (Baijia Biotechnology Co., Ltd., IPB0062); Rabbit anti-interleukin 4 (Il-4) polyclonal antibody (Baijia Biotechnology Co., Ltd., IPB3904); Rabbit anti-interleukin 10 (Il-10) polyclonal antibody (Baijia Biotechnology Co., Ltd., IPB0104); Rabbit anti-Tgfb1 polyclonal antibody (ABclonal, A16640); Rabbit anti-nitric oxide synthase 2 (Nos2) polyclonal antibody (Proteintech, 22226-1-AP); Rabbit anti-glyceraldehyde-3-phosphate dehydrogenase (GAPDH) polyclonal antibody (Proteintech, 10494-1-AP); Rabbit anti-Alk1 polyclonal antibody (Huabio, HA722529); Rabbit anti-Alk5 polyclonal antibody (Immunoway, YM8858); Rabbit anti-Tgfbr2 polyclonal antibody (Immunoway, YM8220); Rabbit anti-Eng polyclonal antibody (Proteintech, 10862-1-AP); Rabbit anti-Mothers Against Decapentaplegic Homolog 1/5 (Smad1/5) polyclonal antibody (immunoway, YT4325); Rabbit anti-Smad2/3 polyclonal antibody (Wanleibio, wl01520); Rabbit anti-phospho-Smad1/5 (p-Smad1/5) polyclonal antibody (Huabio, HA722566); Rabbit anti-p-Smad2/3 polyclonal antibody (Wanleibio, WL02305); Rabbit anti-DYKDDDDK-tag monoclonal antibody (Proteintech, 20543-1-AP); Rabbit anti-His-tag polyclonal antibody (Proteintech, 10001-0-AP); Rabbit anti-HA-Tag(26D11) polyclonal antibody (Proteintech, 51064-2-AP); Rabbit anti-myelocytomatosis oncogene (Myc)-tag monoclonal antibody (Abcom, ab9106); Rabbit anti-glutathione S-transferase (GST)-tag monoclonal antibody (ABclonal, AE006); and Rabbit anti-V5-tag polyclonal antibody (Proteintech, 14440-1-AP).

    Techniques: Knock-Out, Western Blot, Activation Assay, Expressing, Staining

    Identification of IGSF8 and IL10RB as new ADAM9 substrates. HCT116 cells were transfected with the indicated siRNA or expression constructs, and Western blotting was performed for conditioned media (CM) or cell lysates (CLs) using antibodies recognizing the ectodomain of IGSF8 ( A , B ) or IL10RB ( C , D ). Three biological replicates are shown in ( A , C ); unpaired two-tailed t test was performed. ADAM, A Disintegrin And Metalloproteinase; IGSF8, immunoglobulin superfamily member 8; IL10RB, interleukin 10 receptor subunit beta.

    Journal: Molecular & Cellular Proteomics : MCP

    Article Title: An Integrative Proteotranscriptomics Approach Reveals New ADAM9 Substrates and Downstream Pathways

    doi: 10.1016/j.mcpro.2026.101538

    Figure Lengend Snippet: Identification of IGSF8 and IL10RB as new ADAM9 substrates. HCT116 cells were transfected with the indicated siRNA or expression constructs, and Western blotting was performed for conditioned media (CM) or cell lysates (CLs) using antibodies recognizing the ectodomain of IGSF8 ( A , B ) or IL10RB ( C , D ). Three biological replicates are shown in ( A , C ); unpaired two-tailed t test was performed. ADAM, A Disintegrin And Metalloproteinase; IGSF8, immunoglobulin superfamily member 8; IL10RB, interleukin 10 receptor subunit beta.

    Article Snippet: Antibodies used in this study include rabbit anti-ADAM9 (CST, 4151, 1:1000 dilution), mouse anti-ALCAM (Santa Cruz, 74558, 1:200 dilution), mouse anti–interleukin 10 receptor subunit beta (IL10RB) (R&D Systems, MAB874, 5 μg/ml), rabbit anti–Forkhead Box O3 (FOXO3) (CST, 2497, 1:1000 dilution for Western blotting, and 1:100 dilution for immunocytochemistry [ICC]), rabbit anti–immunoglobulin superfamily member 8 (IGSF8) (Sigma, 011917, 1:500 dilution), horseradish peroxidase (HRP)–conjugated rabbit anti-mouse (CST, 7076, 1:2000 dilution), HRP-conjugated goat anti-rabbit (CST, 7074; 1:2000 dilution), HRP-conjugated mouse anti-β-actin antibody (CST, 12262, 1:2000 dilution), and Alexa Fluor 594–conjugated goat anti-rabbit antibody (Thermo Fisher, A-11012, 1:1000 dilution for ICC).

    Techniques: Transfection, Expressing, Construct, Western Blot, Two Tailed Test

    Detection results of IL-10 in skin wound area; NC: regular control group; PN: Panax notoginseng group; A(1, 2)∼F(1, 2): Immunohistochemical staining of IL-10 in skin wound area; G: relative mRNA expression level of IL-10; H: IL-10 protein expression level detected by ELISA; Comparison between PN and NC, * P<0.05; **P<0.01

    Journal: bioRxiv

    Article Title: Exploring the mechanism of Panax Notoginseng in the treatment of skin wound based on network pharmacology and experimental verification

    doi: 10.64898/2026.02.28.708691

    Figure Lengend Snippet: Detection results of IL-10 in skin wound area; NC: regular control group; PN: Panax notoginseng group; A(1, 2)∼F(1, 2): Immunohistochemical staining of IL-10 in skin wound area; G: relative mRNA expression level of IL-10; H: IL-10 protein expression level detected by ELISA; Comparison between PN and NC, * P<0.05; **P<0.01

    Article Snippet: Interleukin-6(IL-6) monoclonal antibody (Article No.: 66146-1-Ig, Proteintech Group,lnc.), Tumor Necrosis Factor -α(TNF-α) monoclonal antibody (Article No.: 60291-1-Ig, Proteintech Group,lnc.), Interleukin-10 (IL-10) monoclonal antibody (Article No.: 60269-1-Ig, Proteintech Group, Inc.).

    Techniques: Control, Immunohistochemical staining, Staining, Expressing, Enzyme-linked Immunosorbent Assay, Comparison

    Ptbp2 modifies microglial polarization by downregulating the NF-κB pathway ( a- e ) Representative western blots of p-p65 and p65 and quantitative analysis of protein levels normalized to GAPDH ( n = 3). ( e-f ) Quantitative analysis of TNF-α levels normalized to GAPDH and representative western blots ( n = 3). ( g-l ) ELISA for evaluating TNF-α and IL-10 levels in the mouse blood, brain tissue, and BV-2 cells ( n = 6). * P < 0.05, ** P < 0.01, *** P < 0.001, **** P < 0.0001

    Journal: Molecular Neurobiology

    Article Title: Ptbp2 Alleviates Neuroinflammation and Blood–brain Barrier Disruption via Modulating Microglial Polarization in Ischemic Stroke

    doi: 10.1007/s12035-026-05704-3

    Figure Lengend Snippet: Ptbp2 modifies microglial polarization by downregulating the NF-κB pathway ( a- e ) Representative western blots of p-p65 and p65 and quantitative analysis of protein levels normalized to GAPDH ( n = 3). ( e-f ) Quantitative analysis of TNF-α levels normalized to GAPDH and representative western blots ( n = 3). ( g-l ) ELISA for evaluating TNF-α and IL-10 levels in the mouse blood, brain tissue, and BV-2 cells ( n = 6). * P < 0.05, ** P < 0.01, *** P < 0.001, **** P < 0.0001

    Article Snippet: TNF-α and IL-10 concentrations were determined using the quick ELISA kits (TNF-α, BOSTER EK0527; IL-10, BOSTER EK0417) following the supplier's instructions.

    Techniques: Western Blot, Enzyme-linked Immunosorbent Assay